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Watch the Full Video at https://www.jove.com/v/4218/human-neu.... Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy - a 2 minute Preview of the Experimental Protocol Chung Wong, Evan Vosburgh, Arnold J. Levine, Lei Cong, Eugenia Y. Xu Raymond and Beverly Sackler Foundation,; University of Medicine and Dentistry of New Jersey, The Cancer Institute of New Jersey; Institute for Advanced Study, Princeton, New Jersey, School of Natural Sciences; We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments