Ensuring Even Blocking of Your Membrane | Fluorescence: Blocking the Membrane скачать в хорошем качестве

Ensuring Even Blocking of Your Membrane | Fluorescence: Blocking the Membrane

Ensuring Even Blocking of Your Membrane Using the same type and amount of blocking buffer for every Western blot is one example of the best practices that will lead to producing consistent Western blot data. Another best practice is to adhere to the same protocol, every time you run a Western blot. To save on reagents you may be tempted to skimp on the amount of blocking buffer or dilute it. However, if your membrane is not entirely covered with blocking buffer or if your blocking agent is too dilute, the membrane will not be entirely blocked. Unblocked regions of the membrane are susceptible to non-specific binding of the primary and secondary antibodies, leading to high background. When adding the blocking solution to your membrane, tilt the incubation box and pour the solution down the inside of the box rather than directly on top of the membrane. About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://www.licor.com/bio/reagents Image Studio™ Software https://www.licor.com/image-studio LinkedIn   / licorbio   Twitter   / licorbio   Facebook   / licorbio