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This is a presentation recorded at the 2023 joint conference of the Society for Economic Botany and Society of Ethnobiology, held at Emory University in Atlanta, Georgia from June 4-9, 2023. #ethnobotany #ethnobiology #science #cannabis #cultivation #biodiversity *** Agrobiodiversity in Cannabis Cultivation: An exploration of cannabis cultivator preferences and practices in Colorado and Oregon Berit Dinsdale Over the last decade an increasing number of states have ratified legislation to legalize the production, sale, and consumption of Cannabis sativa, creating one of the most lucrative agricultural industries within the United States. Little research to-date, however, has explored cannabis breeding and cultivation activities, and their influence on cannabis biodiversity. The topic of this paper addresses this understudied area of inquiry by utilizing qualitative methodologies (free-listing and interviews) to assess the preferences, practices, and knowledge of cannabis cultivators and breeders in the states of Oregon and Colorado, and how these elements are applied within the cannabis cultivation and breeding process. Free listing results from this study identified 52 unique attributes of interest to growers and breeders. Of these attributes, yield (horticultural) ranked the highest both in terms of frequency and salience, followed by High THC content (chemometric), and smell or aroma (horticultural). While yield and high THC were the two most salient characteristics in terms of grower preferences, qualitative interviews clarified that both attributes were valued less due to producer's personal preferences than to market demands and remaining competitive. These results indicate that while cannabis grower and breeders may be motivated in part, by individual preferences and practices, external institutions and markets play a key role in influencing cannabis production and potentially, overall cannabis biodiversity.