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This video "Pharmacological Lab Procedures: Maintenance of an Isolated Smooth Muscle Preparation in Vitro: Calcium-Free Physiological Solution" is part of a multimedia-based module by George Koffour, Samuel Owusu Agyeman-Duah, Benjamin Prempeh, and Ella Kasanga. This module contains a series of demonstration videos on how to do isolated smooth muscle preparation in vitro using the Harvard Kymograph simulator. The complete learning module is available through the African Health OER Network at: http://www.oerafrica.org/healthoer/Ho... Any medical information in this material is intended to inform and educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. Please speak to your physician if you have questions about your medical condition. Viewer discretion is advised: Some medical content is graphic and may not be suitable for all viewers. This is licensed under a Creative Commons Attribution License: http://creativecommons.org/licenses/b.... (c) 2010-11 Kwame Nkrumah University of Science and Technology. Any medical information in this material is intended to inform and educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. Please speak to your physician if you have questions about your medical condition.