Which RT-PCR Method to Choose? One-Step vs Two-Step скачать в хорошем качестве

Which RT-PCR Method to Choose? One-Step vs Two-Step

Choosing between one-step and two-step RT-PCR methods is essential for successful cDNA synthesis. In this video from Thermo Fisher Scientific, learn how each method works, when to use them, and how reverse transcription integrates with PCR. Understand the advantages of one-step RT-PCR for speed and reduced contamination, and why two-step RT-PCR is ideal when flexibility or multiple targets are needed. Chapters: 00:00 Introduction to RT-PCR 00:15 Two-Step RT-PCR Process 00:45 One-Step RT-PCR Process 01:10 Advantages of One-Step RT-PCR 01:35 Advantages of Two-Step RT-PCR 02:00 Applications in Research and High-Throughput Workflows Thermo Fisher Scientific provides trusted reverse transcription and PCR solutions to support your molecular biology research. 3. Thumbnail Concepts Thumbnail 1: Split-screen design showing two workflows: left panel with two tubes labeled 'Step 1: RT' and 'Step 2: PCR', right panel showing a single tube labeled '1-Step RT-PCR'. Overlay text: 'One-Step vs Two-Step RT-PCR'. Bold Helvetica Neue font, Thermo Fisher red accent, clean lab-inspired background. (≈500 chars) Thumbnail 2: Simplified infographic of RNA strand being converted to cDNA with reverse transcriptase, then amplified with DNA polymerase. Overlay text: 'cDNA Synthesis Guide'. White and dark gray background with Thermo Fisher branding colors for clarity and professional tone. (≈500 chars) Thumbnail 3: Illustration of a researcher choosing between two workflows (two-step vs one-step), represented with icons of tubes and pipettes. Overlay text: 'Which RT-PCR Method?'. Bold Helvetica Neue text, navy and red accents, strong diagonals to imply decision-making. (≈500 chars)