Marker Verification for Mass Cytometry скачать в хорошем качестве

Marker Verification for Mass Cytometry

For every marker on our cytometry panels, we perform a six-point titration to identify the optimal antibody concentration for staining. This YouTube video will take you through our step-by-step approach to interpreting the results of these titrations ensuring optimal staining of surface, cytoplasmic, and intra-nuclear proteins by cytometry. As a preview, here are some of the key metrics we consider to select the ideal concentration of each antibody: ✅Staining Index: High SI values help to clearly separate positive and negative populations. ✅Median Channel Values: MCVs within the 10 – 100 range minimize signal spillover into other channels. ✅Spillover: Near-zero spillover values reduce false positive signals for other markers on the panel. By optimizing these parameters, we ensure accurate and reproducible cytometry results. 💡Curious to see this in action? In this video, we’ll demonstrate this process using three example markers: CD25, Granzyme B, and Tbet. 0:00 Introduction 0:24 Marker Verification Protocol 2:20 CD25 3:47 Granzyme B 5:00 Tbet 6:17 Read More Like what you see? Follow us for more topics in cytometry!   / teiko-bio     / teikobio