Effector CD8+ T (CD8 TE) cells crawl along liver sinusoids скачать в хорошем качестве

Effector CD8+ T (CD8 TE) cells crawl along liver sinusoids

For more information, visit: https://www.miltenyibiotec.com/produc... Crawling of effector CD8+ T (CD8 TE) cells visualized by multiphoton intravital microscopy in the liver of a HBcAg transgenic mouse (H2b) that was injected with H2b-restricted Cor93 (red) and H2d-restricted Env28 (green) CD8 TE. Sinusoids are in gray and hepatocellular nuclei are in blue. Scale bar represents 50 mm. Observations indicate that CD8 TE exhibit an intrasinusoidal crawling behavior that halts when hepatocellular antigens are recognized. This is particularly relevant for the pathogenesis of viral infections such as those caused by HBV and HCV. Light sheet microscopy allows for fast high-resolution imaging of 3D biological samples, like whole rodent organs, embryos, or larvae. The use of paired orthogonal optical pathways to confine illumination to a single plane allows researchers to image sample volumes faster while limiting light dosage. This technique opens new avenues to study entire biological systems and processes in physiologically representative 3D samples. Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our more than 25 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has more than 2,500 employees in 28 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health. [courtesy of Matteo Iannacone, San Raffaele Scientific Institute, Milan, Italy]