Identification: Protein Interaction Partners In Mammalian Cells By SILAC l Protocol Preview скачать в хорошем качестве

Identification: Protein Interaction Partners In Mammalian Cells By SILAC l Protocol Preview

Watch the Full Video at https://www.jove.com/v/51656/identifi.... Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics - a 2 minute Preview of the Experimental Protocol Edward Emmott, Ian Goodfellow University of Cambridge, Division of Virology, Department of Pathology; SILAC immunoprecipitation experiments represent a powerful means for discovering novel protein:protein interactions. By allowing the accurate relative quantification of protein abundance in both control and test samples, true interactions may be easily distinguished from experimental contaminants, and low affinity interactions preserved through use of less-stringent buffer conditions. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments