Multiplex Immunoassay Detection of Alzheimer's Disease Biomarker скачать в хорошем качестве

Multiplex Immunoassay Detection of Alzheimer's Disease Biomarker

Presented At: Neuroscience Virtual Event 2018 Presented By: Anthony Saporita, PhD Senior Scientist, MilliporeSigma Speaker Biogrpahy: Dr. Saporita has over 15 years of experience in protein biochemistry and assay development. He earned his B.S. from University of Missouri and Ph.D. from Northwestern University and received postdoctoral training at Washington University in Saint Louis. As part of the Research & Development team at MilliporeSigma, Dr. Saporita designs new immunoassay kits for protein biomarker detection for several research categories including neuroscience, toxicity, and cell signaling. Webinar: Multiplex Immunoassay Detection of Alzheimer's Disease Biomarkers in Cerebrospinal Fluid, Plasma, and Serum Webinar Abstract: Monitoring protein biomarkers in cerebrospinal fluid (CSF) of patients with Alzheimer’s Disease (AD) has been highly beneficial to understanding disease progression. While several CSF biomarkers can reproducibly distinguish normal and diseased samples, CSF is a difficult biological fluid to obtain in research studies. The need for blood-based biomarkers of AD has driven a continuous search for novel candidates. Here we report the development of a multiplex immunoassay to quantitatively measure seven proteins present in both CSF and blood that are involved in neurological disease: Neurogranin, TREM2, ApoE4, FABP3, Ferritin, angiogenin, and prion protein. Notably, presence of the APOE4 allele is prominently associated with an increased risk for AD, in comparison to the APOE2 and APOE3 alleles. Although these ApoE isoforms differ at only one or two amino acids, our assay distinguished ApoE4 with minimal cross-reactivity. Using this novel immunoassay, we measured these 7 biomarkers in CSF, plasma, and serum from AD patients and healthy controls. Additionally, we developed an ultrasensitive Single Molecule Counting (SMC) assay for amyloid beta 1-42 (Aβ42). This kit could detect the Aβ42 peptide in CSF and plasma at sub-pg/mL concentrations. This study demonstrates the value of evaluating both novel and established biomarkers of neurodegeneration across distinct sample types. Earn PACE/CME Credits: 1. Make sure you’re a registered member of LabRoots (https://www.labroots.com/virtual-even...) 2. Watch the webinar on YouTube above or on the LabRoots Website (https://www.labroots.com/virtual-even...) 3. Click Here to get your PACE (Expiration date –March 14, 2020 06:00 AM)– http://www.labroots.com/credit/pace-c... LabRoots on Social: Facebook:   / labrootsinc   Twitter:   / labroots   LinkedIn:   / labr.  . Instagram:   / labrootsinc   Pinterest:   / labroots   SnapChat: labroots_inc