P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality скачать в хорошем качестве

P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality

References/Resources:   / the_crux   This video discusses the general biology of P1 bacteriophage based cloning vectors (pNS582 and pAd10SacBII) and P1 derived artificial chromosomes (PACs - pCYPAC1). The discussion walks you through the development of the P1 system with its use of Pac site and Cre-loxP recombination, coupled with SacB driven synthetic lethality. The main focus of the video is understanding Pac site packaging, Cre-loxP recombination and how they are used in P1 vectors (pNS582 and pAd10), along with SacB gene for counter selection. The P1 vectors are interesting in themselves because they use a cryptic origin of replication (P1 lytic origin) to increase the copy number of a vector. PACs specifically benefit from this origin, helping increase the overall vector yield in DNA extraction. Jump to your favorite section: 00:00 P1 bacteriophage 01:19 pNS582 03:50 Cre-LoxP 05:38 Using P1 vectors 08:50 P1 Lytic origin 10:10 Disadvantages 11:58 pAd10SacBII 12:38 Using SacB for selection 14:02 PACs - pCYPAC1 Patreon Page:   / the_crux   Suggested videos: Plasmids:    • Plasmid and Phagemid cloning vector - Sele...   Lambda Phage and Cosmids:    • Lambda Phage vector, Cosmid cloning vector...   Fosmids/BACs:    • F plasmid, BACs, Fosmid cloning vector - F...   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Consider supporting the growth of this channel by becoming a Patron:   / the_crux   I appreciate your support :)