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Watch the Full Video at https://www.jove.com/v/56557/applicat.... Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis - a 2 minute Preview of the Experimental Protocol Junhua Lv, Feng Liu Chinese Academy of Sciences, State Key Laboratory of Membrane Biology, Institute of Zoology; University of Chinese Academy of Sciences,; This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments