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Before you can use a new LC column to perform analyses, there are a few important steps you need to do to prepare it for its new life in your lab. In this Restek Tip, we show you how to set up your new LC column. We cover certificate of analyses (CofAs), discuss the different column end-fittings you can use, share how to condition your column for your mobile phase, and finally, we’ll look at how to select an appropriate flow rate for your column. Let’s get started! ----------------------------------------------------------------- Contents 0:00 Preparing a New LC Column 0:46 LC Certificates of Analysis 1:20 Connecting Your LC Column 3:50 Conditioning Your LC Column 4:46 Selecting an Appropriate Flow Rate ----------------------------------------------------------------- Additional Resources Restek Documentation Search | https://www.restek.com/documentation-... Solvent Miscibility and Solubility Chart | https://www.restek.com/articles/solve... Restek LC Column Use and Care Instruction Sheet | https://www.restek.com/articles/reste... HPLC and UHPLC FAQ | https://www.restek.com/articles/frequ... ----------------------------------------------------------------- Restek Recommended Tools & Parts EXP Reusable Fittings | https://www.restek.com/p/25937 Restek’s HPLC & UHPLC Columns | https://www.restek.com/c/1119 Restek’s LC Accessories LC-MS Supplies | https://www.restek.com/c/1122 LC Autosampler Supplies | https://www.restek.com/c/1118 Mobile Phase Management | https://www.restek.com/c/1123 LC Detector Supplies | https://www.restek.com/c/1120 LC Tubing & Fittings | https://www.restek.com/c/1121 LC Pump Supplies | https://www.restek.com/c/1124 Tools & Organization | https://www.restek.com/c/1125 ----------------------------------------------------------------- Connect with Us Comment on this video and subscribe to Restek's YouTube channel today at / restekcorp Website: https://www.restek.com LinkedIn: / restek-corporation Facebook: / restekcorporation