Colorectal Cancer Cell Surface Protein Profiling Using Antibody Microarray l Protocol Preview скачать в хорошем качестве

Colorectal Cancer Cell Surface Protein Profiling Using Antibody Microarray l Protocol Preview

Watch the Full Video at https://www.jove.com/v/3322/colorecta.... Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing - a 2 minute Preview of the Experimental Protocol Jerry Zhou, Larissa Belov, Michael J. Solomon, Charles Chan, Stephen J. Clarke, Richard I. Christopherson University of Sydney, School of Molecular Bioscience; Royal Prince Alfred Hospital, Department of Surgery; Department of Anatomical Pathology, Department of Anatomical Pathology; Concord Repatriation General Hospital, Department of Medicine; We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments