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Watch the Full Video at https://www.jove.com/v/56253/preparat.... Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture - a 2 minute Preview of the Experimental Protocol Yongsan Li, Yaling Zhang, Yen Wei, Lei Tao Tsinghua University, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry; China Academy of Engineering Physics, Institute of Chemical Materials; Here we describe a facile preparation of chitosan-based injectable hydrogels using dynamic imine chemistry. Methods to adjust the hydrogel’s mechanical strength and its application in 3D cell culture are presented. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments