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Watch the Full Video at https://www.jove.com/v/60486/a-human-.... A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk - a 2 minute Preview of the Experimental Protocol Carmen G. Flesher, Nicki A. Baker, Clarissa Strieder-Barboza, Dominic Polsinelli, Phillip J. Webster, Oliver A. Varban, Carey N. Lumeng, Robert W. O'Rourke University of Michigan Medical School, Department of Surgery; University of Michigan Medical School, Department of Pediatrics and Communicable Diseases; University of Michigan Medical School, Graduate Program in Immunology; University of Michigan Medical School, Graduate Program in Cellular and Molecular Biology; University of Michigan, Undergraduate Research Opportunity Program; Ann Arbor Veterans Affairs Healthcare System, Department of Surgery; We describe a 3D human extracellular matrix-adipocyte in vitro culture system that permits dissection of the roles of the matrix and adipocytes in contributing to adipose tissue metabolic phenotype. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments