У нас вы можете посмотреть бесплатно Animated PCR Real Time (qPCR) procedure или скачать в максимальном доступном качестве, видео которое было загружено на ютуб. Для загрузки выберите вариант из формы ниже:
Если кнопки скачивания не
загрузились
НАЖМИТЕ ЗДЕСЬ или обновите страницу
Если возникают проблемы со скачиванием видео, пожалуйста напишите в поддержку по адресу внизу
страницы.
Спасибо за использование сервиса ClipSaver.ru
Quantitative PCR (qPCR) or real time PCR is a form of polymerase chain reaction (PCR) used to amplify and simultaneously detect the amplification product of nucleic acids. This is achieved by incorporating fluorescent molecules that associate with the amplified nucleic acids, so that the increase in fluorescence is proportional to the increase in the amount of amplified DNA/RNA molecules in the reaction. It is a simple and reliable technique that can be applied to different fields in modern biology and related sciences. Real-time PCR can be used for the diagnosis of diseases, such as infectious diseases, cancer and genetic abnormalities. The introduction of quantitative PCR assays in microbiology laboratories has improved the diagnosis of infectious diseases and is also used as a tool to detect new emerging diseases. Real-time PCR is performed in a thermal cycler capable of shining a beam of light of a specific wavelength on each sample and detecting the fluorescence emitted by the excited fluorochrome. This thermal cycler is an apparatus capable of rapidly heating and cooling the samples to take advantage of the physico-chemical qualities of the nucleic acids and the enzymatic qualities of the DNA polymerase. The PCR process usually consists of a series of temperature changes repeated 25-40 times, called cycles, each with a minimum of three steps: the first allows the separation of the double-stranded nucleic acids; the second allows the binding of primers and fluorophore-labelled probes to the DNA strand; the third facilitates polymerisation by the DNA polymerase. The video shows how the real-time PCR reaction takes place on a molecular level, so that the technique can be understood in a simple way. For more info about our qPCR panel, visit: https://operon.es/molecular-diagnostics/