A thin-skull window approach for chronic in vivo two-photon imaging of murine microglia скачать в хорошем качестве

A thin-skull window approach for chronic in vivo two-photon imaging of murine microglia

Reference: https://app.jove.com/t/2059/a-thin-sk... The Thin-skull Window Technique represents a significant advancement in the field of neurobiology, particularly in the study of microglia, the resident immune cells of the central nervous system. This technique has been specifically designed to enable chronic in vivo imaging of murine microglia through the use of two-photon microscopy, a powerful imaging modality that allows for high-resolution visualization of cellular structures and dynamics deep within living tissues. One of the key advantages of the Thin-skull Window Technique is its ability to facilitate long-term observation of microglial behavior in real-time. Traditional imaging methods often require the removal of the skull or other invasive procedures that can alter the natural environment of the brain and affect cellular behavior. In contrast, the Thin-skull Window Technique preserves the integrity of the skull while providing a transparent window through which researchers can directly observe microglial activity over extended periods. This is particularly important in models of neuroinflammation, where understanding the temporal dynamics of microglial responses is crucial for elucidating their roles in various pathological conditions. By utilizing this innovative approach, researchers can gain valuable insights into the cellular dynamics of microglia, including their activation states, morphological changes, and interactions with other cell types in the brain. These observations can reveal how microglia respond to neuroinflammatory stimuli, such as injury, infection, or neurodegenerative diseases, and how these responses may contribute to the progression or resolution of neuroinflammation.