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Watch the Full Video at https://www.jove.com/v/61654/quantify.... Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography - a 2 minute Preview of the Experimental Protocol C. Ryan Oliver, Trisha M. Westerhof, Maria G. Castro, Sofia D. Merajver University of Michigan Ann Arbor, Department of Internal Medicine; University of Michigan Ann Arbor, Rogel Cancer Center; University of Michigan Ann Arbor, Department of Neurosurgery; University of Michigan Ann Arbor, Department of Cell and Developmental Biology; Here, we present a protocol for preparing and culturing a blood brain barrier metastatic tumor micro-environment and then quantifying its state using confocal imaging and artificial intelligence (machine learning). Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments