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In this video, I connect your absorbance vs. concentration graph to Beer’s Law and explain what the trendline equation actually means. 00:00 Intro – How your lab graph shows Beer’s Law 00:29 Slope-intercept form: y = mx + b 00:46 Beer’s Law: A = εbc 01:04 Why absorbance is y and concentration is x 01:33 What the trendline slope means (ε × path length) 01:55 Why path length is usually 1 cm 02:21 Why the slope equals ε 02:38 What the y-intercept really means 03:25 Determining molar absorptivity from the graph 04:12 Using the trendline to find an unknown concentration We’ll compare the slope-intercept form of a line (y=mx+b) to Beer’s Law (A=εbc) so you can clearly see: 🧪 Why absorbance is plotted on the y-axis 🧪Why concentration is on the x-axis 🧪What the slope of the graph represents 🧪Why your trendline has a y-intercept, even though Beer’s Law doesn’t This video is a continuation of my previous lesson on plotting absorbance vs. concentration, and it’s especially helpful for general chemistry and biochemistry lab students who are confused about calibration curves and Beer’s Law. 💡 By the end, you’ll understand how your experimental data connects directly to the theory behind Beer’s Law - and how to interpret your graph with confidence. #BeersLaw, #GeneralChemistry, #ChemistryLab, #Absorbance, #CalibrationCurve, #Biochemistry, #ChemHelp