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Watch the Full Video at https://www.jove.com/v/58117/plate-ba.... Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes - a 2 minute Preview of the Experimental Protocol Katharina Kohl, Thomas Fleming, Kübra Acunman, Hans-Peter Hammes, Michael Morcos, Andrea Schlotterer Heidelberg University, 5th Medical Department, Medical Faculty Mannheim; Heidelberg University, Department of Internal Medicine; German Center for Diabetes Research (DZD),; Heidelberg University, European Center for Angioscience, Medical Faculty Mannheim; This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments