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Watch the Full Video at https://www.jove.com/v/59402/high-res.... High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue - a 2 minute Preview of the Experimental Protocol Luca Zaeck, Madlin Potratz, Conrad M. Freuling, Thomas Müller, Stefan Finke Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut; Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments