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Agarose Gel Electrophoresis

Demonstration of a 0.8% (w/v) Agarose gel loading with ladder, uncut plasmid & restriction enzyme cut plasmid. Demonstration starts at the powered Agarose and end with a labelled figure for publication. 0.8% (w/v) Agarose Gel 0.8 Agarose in 100 mL 1X TAE Buffer Microwave for 2 min (until just starts to boil) @ 70% power Addition of 5 uL Red Safe Pour when you can hold it in your hand Fill chamber with 1X TAE Buffer Wells were loaded from left to right by: 1 Kb ladder Uncut Plasmid DNA (pUC:URA) EcoRI cut Plasmid DNA HindIII cut Plasmid DNA HindIII + EcoRI cut Plasmid DNA Red Safe used for viewing