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Join us to discuss cutting-edge technologies and advances in the biomedical and life sciences! __ In this webinar, we explore advancements in understanding DNA replication dynamics at the single-cell level, focusing on the innovative method of single-cell 5-ethynyl-2′-deoxyuridine sequencing (scEdU-seq). DNA replication, a vital process ensuring accurate genome duplication before cell division, varies across different epigenetic states and cell types. Using scEdU-seq, researchers can now measure replication speeds with high precision and observe how these speeds fluctuate during various stages of the S phase. Join us as we delve into the temporal variations in DNA replication speed, the role of transcription-induced DNA damage in limiting replication speed, and the implications for developmental defects and tumorigenesis. The webinar will also highlight key genetic and pharmacological perturbations that influence DNA replication, offering insights into the complex interplay between replication dynamics and genomic integrity. Highlights 📌 Introduction to scEdU-seq: A novel method for measuring DNA replication speed in single cells. 📌Insights into the variability of replication speed during the S phase of the cell cycle. 📌The impact of transcription-induced DNA damage on replication dynamics. 📌Discussion on the acceleration of replication in the late S phase. 📌Applications of genetic and pharmacological perturbations to modulate replication speed. 📌Implications for understanding developmental defects and cancer development. 📄 Read more ➡ van den Berg, J., van Batenburg, V., Geisenberger, C. et al. Quantifying DNA replication speeds in single cells by scEdU-seq. Nat Methods 21, 1175–1184 (2024). https://doi.org/10.1038/s41592-024-02... ❓ Share your question in the webinar Q&A ➡https://bio-protocol.org/webinar/deta... __ 🥼 About Bio-protocol Webinar Series The Bio-protocol webinar series highlights technical advances in biomedical and life science, focusing on a detailed presentation of cutting-edge technologies and techniques. We aim to make this program a go-to place for sharing, learning, discussing, and uncovering how to apply new technologies and tools in the life science community. 📩 To stay updated on our new webinars ➡ https://bio-protocol.org/webinar If this video was helpful, don't forget to like, subscribe, and share this video! For inquiries, contact us at: [email protected] Follow Bio-protocol! 🌿 Bluesky ➡https://bsky.app/profile/bio-protocol... 🔬 LinkedIn ➡ / bio-protocol