Polycystic Kidney Disease: Knockdown of wnt5a Causes | Protocol Preview скачать в хорошем качестве

Polycystic Kidney Disease: Knockdown of wnt5a Causes | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52156/a-possib.... A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys - a 2 minute Preview of the Experimental Protocol Liwei Huang, An Xiao, Andrea Wecker, Daniel A. McBride, Soo Young Choi, Weibin Zhou, Joshua H. Lipschutz Eastern Virginia Medical School, Department of Medicine; Medical University of South Carolina, Department of Medicine; University of Michigan, Department of Pediatrics; We describe a method of generating a possible zebrafish model of polycystic kidney disease. We used Tg(wt1b:GFP) fish to visualize kidney structure. Knockdown of wnt5a was by morpholino injection. Pronephric cyst formation after wnt5a knockdown was observed in this GFP transgenic zebrafish. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments