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Watch the Full Video at https://www.jove.com/v/705/single-cel.... Single Cell Electroporation in vivo within the Intact Developing Brain - a 2 minute Preview of the Experimental Protocol D. Sesath Hewapathirane, Kurt Haas University of British Columbia - UBC, Brain Research Centre; University of British Columbia - UBC, Department of Cellular and Physiological Sciences; Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments