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This session was presented at the 2024 World Congress on In Vitro Biology Meeting held in St. Louis, Missouri from June 8 - 12, 2024 and was recorded as part of the In-Person and On-Demand Limited Access Program. Plant genetic engineering and gene editing typically start with a reliable plant transformation system to introduce desirable traits into crops of interest. The success of plant transformation depends on the ability of transformed cells to regenerate and develop into plants with heritability. Despite significant progress made in understanding the underlying cellular and molecular mechanisms, many important plant species and germplasms remain recalcitrant to transformation and regeneration process. Among several transformation methods currently available, Agrobacterium mediated transformation is still a lead method with still relatively low efficiency and limited by the host-microbe interaction, which hinders the crop improvement programs. To address challenges in crops and germplasms limitation, low efficiency of Agrobacterium mediated transformation, researchers have been devoted in developing new and improving transformation processes. This session will cover recent advances in plant transformation method developments, including Agrobacterium engineering, improving transformation rates, simultaneous delivery to multiple germplasms, and recovery of transgene free gene edited plants. This work is supported by Agriculture and Food Research Initiative [grant no. 2022-67013-37019/project accession no. 1028426] from the USDA National Institute of Food and Agriculture. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. Learn more about the SIVB Annual Meetings at https://sivb.org/meetings.