Histidine-Tagged Protein Capture Using the ProteOn™ XPR36 System скачать в хорошем качестве

Histidine-Tagged Protein Capture Using the ProteOn™ XPR36 System

For more info, visit http://www.bio-rad.com/yt/11/proteonw.... Jay Duffner, a senior scientist at Momenta Pharmaceuticals, presents techniques for improving results in the measurement of binding kinetics of antibodies to histidine-tagged protein targets using the ProteOn system. Techniques discussed include capture conditions for histidine-tagged proteins, the reduction of non-specific binding, chip regeneration techniques, and measurement of ligand activity over the course of an assay. The ProteOn HTG sensor chip is functionalized with a novel tris-NTA complex for improved capture of histidine-tagged proteins. This tris-NTA complex is unique to the ProteOn HTG and HTE chips and provides excellent binding stability and regeneration capability in the capture of histidine-tagged proteins. The HTG chip has a compact binding capacity and is most suitable for protein-protein and protein-peptide interaction analysis. Features and Benefits: • Full compatibility with the ProteOn XPR36 protein interaction array system • Improved binding capacity and stability due to the novel tris-NTA formulation • Reduced decay of ligand over time -- ensures reliable results and no cumbersome software corrections are needed • Capture of histidine-tagged proteins directly from crude media, reducing work-up labor and required sample volumes • Enhanced efficiency and reduced costs due to surface regeneration ability (typically 10 or more surface regeneration cycles) • Surface regeneration combined with One-shot Kinetics™ technology yields exceptionally high throughput http://www.bio-rad.com/evportal/desti... We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn:   / 1613226   Facebook:   / biorad   Twitter:   / bioradlifesci   Instagram: @BioRadLabs Snapchat: @BioRadLabs