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Genetic Testing of Cannabis to Determine Genotype

Genetic Testing of Cannabis to Determine Genotype What is PCR? PCR = Polymerase Chain Reaction Scientific process that allows for small (theoretically 1) sequence of DNA to be replicated into many copies Having multiple copies can identify if the sequence was present This is a very precise process Genetic Testing Options Male/Female Genotype Sequencing IP (Intellectual Property) protection Research Study Mendel, Peter & Lalge, Ajinkya & Vyhnánek, Tomáš & Trojan, Václav & Kalousek, Petr & Maassen, Hugo & Havel, Ladislav. (2016). PROGRESS IN EARLY SEX DETERMINATION OF CANNABIS PLANT BY DNA MARKERS. https://www.researchgate.net/publicat... Gender Detection Screening of Cannabis DNA Sex/Gender Testing Benefits Saving time by discovering if a plant is male months before it could be traditionally (phenotypically) detected. Saving resources by identifying plants early fewer plants need to be grown which reduces the inputs (water, labor, nutrients) that are needed and maximizes the space to grow plants in. Upfront cost for testing can be justified by the reduced cost of reduced plant care. Overview of Samples Four well known varieties of industrial hemp and six experimental varieties of medicinal cannabis were included in this experiment 3 Different Primer Pairs Used for Sex Determination Not all have the same reliability… Visualization of PCR products for sex determination of cannabis varieties (MADC2 marker) Study Conclusions The SCAR119 marker appeared to be the most reliable for male plant detection in cannabis. Other two showed limited or even controversial results This study confirmed that sex determination of the cannabis plant is a complex process. Cannabis Genotyping The genotype is essentially the plants blue print which includes the entire spectrum of possibilities for a particular cannabis plant. Same can be said for the human genome Knowing precisely what defined characteristics are in the plant will help guide decision making when it comes to breeding. Cannabis Sequencing Can be used to determine stability and uniqueness of a cannabis plant while also determining relatives, origin, and ancestral lineage. Once the genetic code is known this can allow for new and unique strains Having record of the genetic sequence can allow for protection against false patent claims and other legal controversies. Sequencing Cannabis Genetics for IP Protection The greater the amount of nucleotide bases sequenced the greater power of differentiation can occur. There are two classifications of sequencing.. Targeted sequencing: Of more than 3.5 million bases (Mb) across thousands of high-value targets, including 29 cannabinoid and terpene synthase genes as well as several genes for seed production and sex determination.  Whole genome sequencing: Provides a data file that contains the strain’s full genetic sequence which can be used to identify important genetic markers and determine novelty. *Since cannabis genome is highly polymorphic so single primer sets can often have reduced overall identification abilities. Link to Lecture Slides: https://drive.google.com/file/d/1A06t... *Due to the description character limit the full work cited for "Genetic Testing of Cannabis to Determine Genotype" can be viewed at... https://drive.google.com/file/d/1T9m1...