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Watch the Full Video at https://www.jove.com/v/54922/efficien.... Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition - a 2 minute Preview of the Experimental Protocol Sanggil Kim, Wooseok Ko, Hyunji Park, Hyun Soo Lee Sogang University, Department of Chemistry; Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC). Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments