Endothelial Cells Isolation, Expandion, and Adipogenic Induction | Protocol Preview скачать в хорошем качестве

Endothelial Cells Isolation, Expandion, and Adipogenic Induction | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57804/isolatio.... Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue - a 2 minute Preview of the Experimental Protocol Bronson A. Haynes, Ryan W. Huyck, Ashley J. James, Meghan E. Carter, Omnia U. Gaafar, Marjorie Day, Avennette Pinto, Anca D. Dobrian Eastern Virginia Medical School, Department of Physiological Sciences; The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments