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Immunoprecipitation is a widely used technique to enrich proteins, peptides, and protein complexes. This video includes a step-by-step demonstration of the Immunoprecipitation protocol for Native Protein, performed by scientists at Cell Signaling Technology. ● CST Immunoprecipitation Protocols: https://www.cellsignal.com/learn-and-... ● Immunoprecipitation Resource Center: https://www.cellsignal.com/applicatio... ● Immunoprecipitation Experimental Design Tips webpage: https://www.cellsignal.com/applicatio... ● CST Proteomics Analytics Services: https://www.cellsignal.com/services/p... Contents: 0:00 Introduction 2:13 Protocol Considerations 3:30 Antibody Selection for Single-Protein IP 4:30 Antibody Selection for Western Blot analysis of IP 4:53 Choice of Beads 6:08 Controls for IP experiments 6:29 Input Control 6:49 Isotype Control 7:12 Bead-Only Control and Unbound Fraction Control 7:46 Lysate Considerations 8:42 A. Solutions and Reagents 10:21 B. Preparing Cell Lysates 12:20 C. Immunoprecipitation 13.37 Lysate Pre-Clearing 17:10 IP Reaction Options 17:32 Unconjugated or Biotinylated Antibodies 18:26 Biotinylated Antibodies + Streptavidin Sepharose Beads 18:44 Unconjugated Antibodies + Magnetic Beads 19:14 Immobilized Antibody-Bead Conjugates 19:46 Tips for Washing Enriched immunocomplexes 22:22 D. Sample Analysis About us: Cell Signaling Technology® (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality, technological innovation, and scientific rigor. We are a company of caring people driven by a devotion to facilitating good science—a company committed to doing the right thing for our Customers, our communities, and our planet. https://cellsignal.com/about All trademarks are the property of their respective owners. For the latest trademark information, visit https://cellsignal.com/trademarks #immunoprecipitation