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Watch the Full Video at https://www.jove.com/v/59677/microman.... Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment - a 2 minute Preview of the Experimental Protocol Cinzia Donato, Barbara M. Szczerba, Manuel C. Scheidmann, Francesc Castro-Giner, Nicola Aceto University of Basel and University Hospital Basel, Cancer Metastasis Laboratory, Department of Biomedicine; SIB Swiss Institute of Bioinformatics,; Here, we present an integrated workflow to identify phenotypic and molecular features that characterize circulating tumor cells (CTCs). We combine live immunostaining and robotic micromanipulation of single and clustered CTCs with single cell-based techniques for downstream analysis and assessment of metastasis-seeding ability. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments