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Watch the Full Video at https://www.jove.com/v/55544/an-effic.... An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production - a 2 minute Preview of the Experimental Protocol Sarah M. Maritan, Eric Y. Lian, Lois M. Mulligan Queen's University Cancer Research Institute, Division of Cancer Biology and Genetics; Queen's University, Department of Pathology and Molecular Medicine; Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments