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Watch the Full Video at https://www.jove.com/v/1343/human-pan.... Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets - a 2 minute Preview of the Experimental Protocol Meirigeng Qi, Barbara Barbaro, Shusen Wang, Yong Wang, Mike Hansen, Jose Oberholzer University of Illinois, Chicago, Department of Surgery; Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part II: purification and culture of human islets) using a modified automated method. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments