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This session was presented at the 2024 World Congress on In Vitro Biology Meeting held in St. Louis, Missouri from June 8 - 12, 2024 and was recorded as part of the In-Person and On-Demand Limited Access Program. This 1-day conference, held on the first day of the World Congress on In Vitro Biology, will focus on current advances in cell/tissue culture technologies and their applications in fish and invertebrate cell culture systems. Technologies presented will include single cell sequencing, RNAi, other genomic/proteomic-based techniques, physiology/toxicology-related approaches, novel imaging methods and medium optimization tools. One area we will highlight is the development of in vitro models that mimic in vivo systems and how these models can be implemented in use-inspired research areas. Key elements of these research areas are the generation of tissue- and species-specific cell culture media and scaffolding systems that mimic the in vivo environment. We will have two sessions, one entitled “Foundational Concepts Underlying Successful Cell/Tissue Culture Strategies” and the other entitled “Current and Future Applications of Invertebrate and Fish Cell Culture Systems.” After these symposia, we will hold a joint panel discussion with speakers and participants from the concurrent Plant Tissue Culture Medium Practices workshop and upcoming mammalian cell culture focused sessions to discuss the major topics from these sessions, including comparing strategies used for cell/tissue culture development and medium optimization. This recording is part 1 of the "Current and Future Applications of Invertebrate and Fish Cell Culture Systems" session. Note: Guy Smagghe's presentation was unable to be included in this recording.