AliveSCENT: A method for engineering E. coli to produce limonene as a natural mosquito repellent скачать в хорошем качестве

AliveSCENT: A method for engineering E. coli to produce limonene as a natural mosquito repellent

Learn more at http://biotreks.org/e202007/ As a vector for disease transmission, mosquitoes are responsible for over 700,000 deaths each year, making it one of the most dangerous animals on the planet. Current methods of repelling mosquitoes, such as DEET, can cause their own problems like skin and eye irritation and allergic reactions. We propose a solution to this public health problem called AliveSCENT, a biologically-inspired mosquito deterrent that relies on the native methylerythritol 4-phosphate (MEP) pathway of Escherichia coli in the production of limonene as a natural, odor-releasing system for commercial use. Limonene is an aromatic compound that makes up a large portion of most citrus scents including lemons and is a safer alternative to sprays and flames and is cleaner than the insect mess created by bug zappers. The smell of the compound by itself has been compared to that of citrus and turpentine, known insect repellents. This product will attempt to reduce the incidence of malaria and other mosquito-borne illnesses. This system will be constructed through plasmid design, assembly, and integration, followed by verification of limonene product using gas chromatography, odor detection by wafting, and evaluation as an effective repellent using a mosquito choice chamber. Here, work towards plasmid assembly will be shown. This phase will require the assembly of a stationary phase promoter, with a ribosome binding site and limonene synthase translational unit into a red fluorescent protein-encoding vector.