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Watch the Full Video at https://www.jove.com/v/4221/repair-cr.... Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate - a 2 minute Preview of the Experimental Protocol David D. Lo, Jeong S. Hyun, Michael T. Chung, Daniel T. Montoro, Andrew Zimmermann, Monica M. Grova, Min Lee, Derrick C. Wan, Michael T. Longaker Stanford University , Department of Surgery; Duke University , Department of Surgery; Saint Joseph Mercy Hospital, Department of Surgery; University of California, San Francisco , School of Medicine; University of California, Los Angeles , School of Dentistry; This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments