25N. Invited Speaker - Laura Waller скачать в хорошем качестве

25N. Invited Speaker - Laura Waller

Computational 3D fluorescence microscopy. November 25th, 2020. by Laura Waller, University of Berkeley (CA, USA) Chair: Genaro Saavedra & Manuel Martinez-Corral Abstract: We describe a compact and inexpensive computational microscope that encodes 3D information into a single 2D sensor measurement, then exploits sparsity to reconstruct the volume with good resolution across a large volume. Our system uses simple hardware and scalable software for easy reproducibility and adoption. The inverse algorithm is based on large-scale nonlinear optimization with self-calibration of aberrations and we discuss computational optical design approaches for optimizing the system’s performance. We demonstrate applications in whole organism bioimaging and neural activity tracking in vivo.