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👇 Full Instructions Below 👇 • Shoots™ and Roots™: https://plantcelltechnology.com/colle... • PPM™: https://plantcelltechnology.com/produ... 🔧 1. Gather Your Materials • 1 sachet of Shoots™ or Roots™ (makes 125 mL) • 125 mL distilled or RO water • 0.5 g Supreme Tissue Culture Agar • 0.25 mL PPM™ (Plant Preservative Mixture) • pH meter or pH paper • Magnetic stirrer or sterile stir rod • Autoclavable flask or bottle • Culture vessels (jars, tubes, or containers) • Autoclave or pressure cooker 💧 2. Dissolve the Media • In an autoclavable flask, add ~100 mL of distilled/RO water. • Pour in the entire Shoots™ or Roots™ sachet (for 125 mL). • Add 0.5 g agar. • Add 0.25 mL PPM™. • Stir gently until everything is fully dissolved. • Add more water to bring the total volume up to 125 mL. ⚖️ 3. Adjust the pH • Measure the pH of the solution. • Adjust to pH 5.7–5.8: • Use small drops of 1N NaOH (to raise pH) • Or 1N HCl (to lower pH) Alternatively, you can use hydroponics pH Up/Down. 🔥 4. Prepare for Sterilization • Loosely cap the flask with the prepared media. • Place your empty culture vessels (jars, tubes, etc.) into the sterilizer as well. • Close the sterilizer but keep the valve open at first to let air escape. ♨️ 5. Media Sterilization • Turn on the sterilizer (autoclave or pressure cooker). • Once steam starts to come out, close the valve. • Sterilize at 121°C (15 psi) for 15–20 minutes. ❄️ 6. Pour Media into Sterile Vessels • Allow sterilized media to cool to around 50–55°C—liquid but not too hot. • In a sterile laminar flow hood, carefully remove the sterile vessels. • Pour the media into vessels inside the hood to avoid contamination. • Let the media cool and solidify inside the vessels. • Close vessels with their sterilized lids. 📝 Key Notes for Success • Sterilize media and vessels together to save time. • Always pour media in a sterile environment (laminar flow hood). • Never place explants into warm media. Label all vessels with: • Date • Medium type (Shoots™ or Roots™) • Plant name or experiment info