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Watch the Full Video at https://www.jove.com/v/55722/differen.... Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells - a 2 minute Preview of the Experimental Protocol Yueying Li, Yong Hai, Jiayu Chen, Tie Liu Capital Medical University, Department of Orthopedics, Beijing Chao-Yang Hospital; Chinese Academy of Sciences, Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics; Tongji University, Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology; We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments