Zeiss LSM800 Airyscan confocal tutorial скачать в хорошем качестве

Zeiss LSM800 Airyscan confocal tutorial

This video covers the hardware and software for Newcastle University's Bioimaging Unit LSM800 confocal, including startup, shutdown, and operating the software. Details and bookmarks for sections below: Startup 00:16 - System Startup 01:52 - Using an Oil Immersion Objective 02:50 - Starting Zen and a quick overview 03:50 - Using the Oculars 04:13 - Using the camera to check your sample Basic channel Setup 05:00 - Setting up for confocal imaging with Smart Setup 07:15 - Describing Tracks and Channels and manual adjustment of Imaging Setup 08:00 - Line and Frame mode differences 09:03 - Detectors on System 09:35 - Fine tuning Imaging Setup manually 11:14- Going Live and using the histogram 11:54 - Using bidrectional scanning 12:15 - Using Nyquist sampling and changing frame size in Acquisition Mode 13:17 - Setting Gain and laser power using the histogram 14:18 - Setting Gain and laser power using the Range indicator 14:38 - 8 bit and 16 bit output 15:05 - Setting gain and laser on a 2 channel Track 15:38 - Taking a Snap Image 16:10 - checking the image for fine tuning 16:54 - Check focal planes for final image 17:34 - Checking for bleedthrough within a multichannel Track 18:40 - Improving Image Quality with dwell time 20:00 - Improving Image Quality with Averaging Zed Stacks 20:47 - Taking Zed Stacks using mark Centre 22:01 - zed sampling frequency 23:10 - Using Start Experiment 23:30 - Viewing 3D images 24:19 - Setting Zed Stack with First and Last Tiling and Multipositions 25:05 - Tiles and Positions setup 26:30 - Using confocal Zoom for overview 26:45 - Taking a Confocal TileScan 27:45 - Taking overview scans using Widefield Imaging 30:15 - Setting Multiple positions from an Overview Scan 31:19 - Checked zed positions for multiposition imaging with Verify Positions 32:14 - Navigating through multiposition czi ifiles 32:30 - Advanced Tiles setup for Preview Scans 34:22 - Setting Support Points for tiled images 36:09 - Focussing Strategy options Confocal and Airyscan imaging 37:38 - Using Confocal Zoom to take cropped images 38:30 - Nyquist confocal Imaging 38:54 - Using Smart Setup to create Airyscan setup 40:40 - comparing Airyscan to Confocal imaging 41:19 - checking Airyscan alignment Timelapse with Mulitpositions 41:50 - moving the stage via captured images to set up multipositional imaging 42:56 - Setting multiposition, multitime imaging 43:26 - Navigating through time series multiposition czi files 43:56 - Setting incubation conditions Saving and Shutting down 44:56 - Saving your images 45:46 - Shutting down Zen 46:02 - Copying data to server Hardware Shutdown 47:05 - Shutting down the hardware 47:22 - Cleaning oil immersion lenses after use