Colóquio: Optical imaging beyond the visible - biomedical applications in the shortwave infrared скачать в хорошем качестве

Colóquio: Optical imaging beyond the visible - biomedical applications in the shortwave infrared

Apresentação: Bernardo A. Arús - National Center for Tumor Diseases (NCT) - Germany Resumo: Biomedical imaging in the shortwave infrared (SWIR, 1000–2000 nm) offers distinct advantages over visible (350–700 nm) and near-infrared (700–1000 nm) wavelengths [1,2]. These benefits include enhanced contrast, lower tissue autofluorescence, and deeper light penetration. Leveraging these SWIR properties, our lab advances optical imaging through both fluorescence and label-free methods [3]. With our collaborators, we have recently developed a library of SWIR fluorescent probes, demonstrating rapid, multiplexed imaging of biological structures in awake, freely moving mice [4,5,6]. Building on these findings, we established a ratiometric fluorescence method that enables quantitative imaging of physiology without anesthesia and restraining, presenting new opportunities for studying organ physiology in freely moving mice [in preparation]. In the label-free front of our research, our SWIR otoscope detects middle ear fluid with superior contrast to its visible counterpart by taking advantage of the strong water absorption at specific SWIR wavelengths [7]. Carrying this idea further, we are constructing a SWIR tissue imager to be utilized in the operating room and pathology lab to detect lymph nodes in adipose tissue, for example. In conclusion, our research demonstrates that exploring wavelengths beyond the conventional ones opens new possibilities for optical biomedical applications. References Carr et al. Proc Natl Acad Sci U S A. 2018, 115(17). doi: 10.1073/pnas.1803210115 Carr et al. Proc Natl Acad Sci U S A. 2018, 115(37). doi: 10.1073/pnas.1718917115 Bruns et al. Nat Biomed Eng. 2017. doi: 10.1038/s41551-017-0056 Cosco et al. Nat Chem. 2020. doi: 10.1038/s41557-020-00554-5 Cosco et al. J Am Chem Soc. 2021. doi: 10.1021/jacs.0c11599 Arús et al. Front Neurosc. 2023. doi: 10.3389/fnins.2023.1135494 Carr et al. Proc Natl Acad Sci U S A. 2016. doi: 10.1073/pnas.1610529113