Quantification-Circulating Pig-Specific DNA In Blood: Xenotransplantation Model l Protocol Preview скачать в хорошем качестве

Quantification-Circulating Pig-Specific DNA In Blood: Xenotransplantation Model l Protocol Preview

Watch the Full Video at https://www.jove.com/v/61579/quantifi.... Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model - a 2 minute Preview of the Experimental Protocol Yangyang Deng, Ming Zhou, Ying Lu, Jiao Chen, Zuhui Pu, Dongjing Yu, Yifan Dai, Yongqiang Zhan, Lisha Mou Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center; Xianning Hospital of Traditional Chinese Medicine,; Liver-biotechnology (Shenzhen) Co., Ltd.,; Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Department of Radiology; University of Toronto, Department of Life Sciences; Nanjing Medical University, Jiangsu Key Laboratory of Xenotransplantation; Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Department of Hepatopancreatobiliary Surgery; In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments