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Watch the Full Video at https://www.jove.com/v/50221/the-use-.... The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers - a 2 minute Preview of the Experimental Protocol Fiach C. O'Mahony, Jyoti Nanda, Alexander Laird, Peter Mullen, Helen Caldwell, Ian M. Overton, Lel Eory, Marie O'Donnell, Dana Faratian, Thomas Powles, David J. Harrison, Grant D. Stewart University of Edinburgh, Edinburgh Urological Cancer Group; University of St Andrews, School of Medicine; University of Edinburgh, Division of Pathology; University of Edinburgh, MRC Human Genetics Unit, MRC IGMM; Western General Hospital, Department of Pathology; University of Edinburgh, Breakthrough Breast Cancer Research Unit; Queen Mary University of London, St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre; RPPA enables the protein expression of hundreds of samples, printed on nitrocellulose slides to be interrogated simultaneously, using fluorescently labelled antibodies. This technique has been applied to study the effect of drug treatment heterogeneity within clear cell renal carcinoma. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments