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Watch the Full Video at https://www.jove.com/v/52233/a-method.... A Method of Nodose Ganglia Injection in Sprague-Dawley Rat - a 2 minute Preview of the Experimental Protocol Michael W. Calik, Miodrag Radulovacki, David W. Carley University of Illinois at Chicago, Center for Narcolepsy, Sleep and Health Research; University of Illinois at Chicago, Department of Pharmacology; University of Illinois at Chicago, Department of Biobehavioral Health Science; Afferent vagal signaling transmits important information to central nervous system from receptors located in organs of the abdomen and thorax. The nodose ganglia of vagus nerves contain many types of receptors that modulate vagal activity. This protocol describes a method of local injections of neurochemicals into the nodose ganglia. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments