Biotechnology Basics: Using the EdvoCycler Jr for Human PCR скачать в хорошем качестве

Biotechnology Basics: Using the EdvoCycler Jr for Human PCR

This is a replay of our biotechnology training workshop from 10/22/20. Biotechnology Basics: Using the EdvoCycler Jr for Human PCR For the slides: https://www.edvotek.com/site/pptx/edv... Featured equipment and experiments: EdvoCycler Jr: https://www.edvotek.com/540 VNTR Human DNA Typing Using PCR: https://www.edvotek.com/334 PCR Model: https://www.edvotek.com/EVT-032 TruBlu™ 2 Transilluminator: https://www.edvotek.com/557 In 1984, Dr. Kary Mullis devised a simple and elegant method to copy specific pieces of DNA known as the polymerase chain reaction (PCR). Because of its ease of use and its ability to rapidly amplify DNA, PCR has become indispensable in the medical and life sciences lab. Today, students can amplify their own DNA in the teaching laboratory, but this can be tricky due to COVID-19 restrictions. In this live stream, we discussed innovations in classroom PCR, including updated DNA extraction protocols and the new EdvoCycler Jr. The concepts will be reinforced using Origami Organelles, which allows students to model the technique using manipulatives. In 1984, Dr. Kary Mullis devised a simple and elegant method to copy specific pieces of DNA known as the polymerase chain reaction (PCR). Mullis was working as a chemist in the DNA synthesis department at the Cetus Corporation. The anecdote was that he was driving along a highway late at night, thinking about DNA synthesis when he came up with the idea that short, synthetic DNA pieces could be used to target a specific DNA sequence. The idea sounded crazy, and my understanding is that Mullis had had several other crazy ideas that did not pan out. After some amount of convincing, Cetus put Mullis and other scientists on this project full time, where through meticulous experimentation, they determined that this technique could in fact amplify DNA. What they found was that this is an incredibly powerful technique that can take the smallest amounts of DNA and expands it to be used for analysis. Because of its ease of use and its ability to rapidly amplify DNA, PCR has become indispensable in the medical and life sciences lab. It’s used in the forensic science lab to create DNA profiles that can be used to identify suspects or victims. And it’s used daily in genetic engineering to amplify pieces of DNA to be spliced into special systems that allow us to make biopharmaceuticals. The technology won Kary Mullis the Nobel Prize in Chemistry in 1993. So, this is pretty amazing stuff. The discovery made in the early 80’s revolutionized molecular biology in many of our lifetimes.