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This is Dr. Frank Rinkevich and I am a Research Entomologist at the USDA-ARS Honey Bee Breeding, Genetics, and Physiology Lab in Baton Rouge, LA. As beekeepers, we all know that Varroa are one of the most important factors that negatively impact honey bee health and contribute to colony losses around the world. Miticide application, especially products containing amitraz, is the most commonly used method to control Varroa populations. However, overuse and over-reliance on amitraz can lead to amitraz resistance in Varroa. Therefore, it is important to understand the patterns and levels of amitraz resistance so we can ensure effective Varroa population management and extend the field use of this important miticide. This video is a step by step set of instructions for you to perform the test for amitraz resistance in Varroa destructor in your honey bee colonies. In this video, we will discuss the items you will receive in the amitraz resistance test kit, the materials that you will need to supply, as well as a demonstration of each individual step in this experiment. Finally, treatment options are suggested based on the results of this experiment. Data sheets and written protocols are available at the USDA-ARS Honey Bee Breeding, Genetics, and Physiology Lab's website. Look over the protocol, datasheet, and video so you can understand exactly what is needed to perform this test. It’s actually much easier to run the test than you might think. You can plan for it to take about a day to complete the test. You’ll need to sample 10 colonies, run the test for 3 hours, count the Varroa and the bees, collect the Varroa in the samples into tubes (we run genetic tests on them), enter in the data, and send it to me for analysis using the contact information below. I’ll go over the results with you shortly after I get the data. If the results are of interest, I will provide a shipping label for you to send me the Varroa you collected in the tubes. If you are interested in participating in this research, I can send you a kit to test your Varroa. I will need an address to ship it and when you think you’d like to run the test. Ideally, it is best to run this test before you apply any miticides. Participation in this study is completely voluntary, unconditional, and anonymous. Previous amitraz use is not required. Data from operations that have not used amitraz provides information on how amitraz resistance may develop and if geography is important for the distribution of amitraz resistance. The only condition is that you share the data with me. All data is good data, even if Varroa populations are low and there is no amitraz resistance. Anonymity is baked into the project. All participants are only known to me and my immediate technicians so we can communicate during sampling and discussing our findings. When communicating with other collaborators and giving presentations, all beekeepers and apiaries that we sample are coded in a way that will never reveal the identity of the source of the data. I am committed to your confidentiality in this process. Let me know if you have any questions about this project. You can contact me at [email protected] or call me at 225-276-3998. I look forward to working with you on this project. Tell anyone else who might be interested if they want to test their Varroa and I can ship them a kit. Best, Frank Rinkevich Research Entomologist USDA ARS Honey Bee Breeding, Genetics, and Physiology Lab 1157 Ben Hur Rd Baton Rouge, LA 70820-5502 Mobile 717-989-2600 Work 225-276-3998 https://www.ars.usda.gov/southeast-area/ba... He/Him/His