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Watch the Full Video at https://www.jove.com/v/61149/a-gnotob.... A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation - a 2 minute Preview of the Experimental Protocol Esther R. Miller, Jonah O'Mara Schwartz, Grace Cox, Benjamin E. Wolfe Tufts University, Department of Biology; A method of growing germ-free Napa cabbages has been developed which enables researchers to evaluate how single microbial species or multispecies microbial communities interact on cabbage leaf surfaces. A sterile vegetable extract is also presented which can be used to measure shifts in community composition during vegetable fermentation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments