BioLamina Rising Stars - Monica Tjin скачать в хорошем качестве

BioLamina Rising Stars - Monica Tjin

Cellular Therapy for Burns/Skin Injuries: Laminin-based Epidermal Keratinocytes Culture System in Xeno-free and Chemically Defined Condition The current expansion of autologous human keratinocytes to resurface severe wound defects still relies on murine feeder layer and calf serum in the cell culture system. Here, the authors report a completely xeno-free and defined culture system utilizing these laminins which enables robust expansion of adult human skin keratinocytes. The authors report a completely xeno-free and defined culture system utilizing these laminins which enables robust expansion of adult human skin keratinocytes, comparable to the 3T3-J2 co-culture system in terms of basal markers’ profile, colony-forming efficiency and the ability to form a normal stratified epidermal structure. The results show that the proposed system may not only provide safer keratinocyte use in the clinics but also facilitate the broader use of other cultured human epithelial cells in regenerative medicine. Monica Suryana Tjin, PhD Duke-NUS Medical School, Singapore