Revolutionary Cloning Made Easy: The Intact Genomics Quick10™ Kit скачать в хорошем качестве

Revolutionary Cloning Made Easy: The Intact Genomics Quick10™ Kit

Introducing the Quick10 Cloning Kit from Intact Genomics. The world's fastest cloning kit. This video introduces the Quick10™ Cloning Kit from Intact Genomics, a revolutionary product that simplifies the cloning process with a 10-minute assembly and transformation process and a 99% success rate. Highlights include: Speed: The entire process takes only 10 minutes, saving researchers valuable time. Ease of use: The kit includes all the necessary components and a simple, step-by-step protocol. Versatility: The kit can be used with your own vectors or single selection, multiple selection, or even custom vectors available from Intact Genomics. High success rate: The kit boasts a 99% success rate, ensuring reliable results. The video then provides a detailed overview of the four main steps involved in the Quick10™ process: Primer design and PCR: This step involves designing primers for your DNA insert and amplifying it using a high-fidelity DNA polymerase. Assembly reaction setup and completion: The diluted PCR product is mixed with the Quick10™ assembly mix and incubated for 7-15 minutes at 37°C. Transformation: The assembly reaction mixture is transformed into competent cells using the direct plate method, eliminating the need for heat shock and outgrowth procedures. Final incubation: The plates are incubated overnight at 37°C for colony growth. The video concludes by emphasizing the convenience and efficiency of the Quick10™ Cloning Kit, and encourages viewers to contact Intact Genomics customer service with any questions. Overall, this video provides a comprehensive overview of the Quick10™ Cloning Kit, making it a valuable resource for researchers looking for a fast, easy, and reliable cloning solution.